Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow-based P-selectin-coated microtube
نویسندگان
چکیده
منابع مشابه
P-Selectin coated microtube for enrichment of CD34+ hematopoietic stem and progenitor cells from human bone marrow.
BACKGROUND Enrichment and purification of hematopoietic stem and progenitor cells (HSPCs) is important in transplantation therapies for hematologic disorders and in basic stem cell research. Primitive CD34+ HSPCs have demonstrated stronger rolling adhesion on selectins than mature CD34- mononuclear cells (MNCs). We have exploited this differential rolling behavior to capture and purify HSPCs fr...
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We have found that the small population of cells in human marrow that are characterized by their expression of CD34 can be readily subdivided into two apparently nonoverlapping subpopulations of approximate equal size, one expressing CD45RO and one CD45R. Functional studies of these subpopulations revealed that all of the primitive erythroid colony-forming cells (BFU-E) are CD34+ CD45RO+. Simil...
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ژورنال
عنوان ژورنال: American Journal of Hematology
سال: 2008
ISSN: 0361-8609,1096-8652
DOI: 10.1002/ajh.21204